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Rev. Soc. Bras. Med. Trop ; 51(1): 88-93, Jan.-Feb. 2018. tab
Article in English | LILACS | ID: biblio-1041448

ABSTRACT

Abstract INTRODUCTION: Here, we determined the genes encoding antibiotic resistance enzymes and virulence factors and evaluated the genetic relationship between Enterobacter spp. isolated from different clinical samples. METHODS: A total of 57 clinical isolates of Enterobacter spp. were tested for the production of extended-spectrum β-lactamases (ESBLs), carbapenemase, and AmpC using phenotypic and genotypic methods. RESULTS: The most common ESBLs and AmpC β-lactamases were bla TEM (63.3%) and bla EBC (57.7%), respectively. The most prevalent virulence gene was rpos (87.7%). The random amplified polymorphic DNA (RAPD) patterns of strains were genetically unrelated. CONCLUSIONS: RAPD polymerase chain reaction analysis revealed high genetic diversity among isolates.


Subject(s)
Humans , Bacterial Proteins/biosynthesis , Bacterial Proteins/genetics , beta-Lactamases/genetics , Escherichia coli/drug effects , Feces/microbiology , Anti-Bacterial Agents/pharmacology , Phenotype , Bacterial Proteins/drug effects , beta-Lactamases/biosynthesis , Polymerase Chain Reaction , Clone Cells , Drug Resistance, Multiple, Bacterial , beta-Lactams/adverse effects , Escherichia coli/enzymology , Escherichia coli/genetics , Disk Diffusion Antimicrobial Tests , Genotype , Iran
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